Assistant Laboratory Animal Technician (ALAT) Practice Exam

Which of these is a common method for euthanizing laboratory animals?

1. Exsanguination

2. CO2 Inhalation

3. Cold exposure

4. Starvation

The correct answer is: CO2 Inhalation

CO2 inhalation is commonly used for euthanizing laboratory animals due to its effectiveness and the control it offers in terms of delivering a humane and quick death. The process involves the animal inhaling carbon dioxide, which acts as an asphyxiant, leading to a loss of consciousness and subsequently death without pain or distress when performed correctly. This method is favored in many protocols due to its relatively fast onset and the ability to administer it in a way that adheres to established animal welfare guidelines. In comparison, while exsanguination can be performed as part of euthanasia in certain contexts, it is typically not a first-line method alone and is often paired with anesthesia to minimize distress. Cold exposure and starvation are not humane methods and could cause undue suffering before death occurs, hence not accepted in regulated research settings.